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KMID : 1138520080110020005
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Journal of Pharmacopuncture
2008 Volume.11 No. 2 p.5 ~ p.12
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Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians
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Sung Baeck-Kyoung
Kim Min-Su
Vyacheslav Ogay
Kang Dae-In
Soh Kwang-Sup
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Abstract
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Objective : In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture.
Methods : 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereoscopic microscope. The specimens were examined by using immunohistochemical methods and were observed under a confocal laser scanning microscope.
Results : A threadlike structure, which was visualized with Alcian blue, existed in dermis layer and proceeded to hypodermis. In this structure, characteristic alignments of rod- shaped nuclei and sized DNA granules were observed. Furthermore, abundant blood capillary plexuses, peripheral nerve endings, and a corpuscle-like structure(about in diameter) were visualized in the skin tissues of acupoints.
Conclusion : It was concluded that the specific threadlike and corpuscle-like structures corresponded to superficial Bonghan duct and corpuscle, respectively.
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KEYWORD
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Acupoint, Intradermal injection, Alcian blue, Tracing, Superficial Bonghan duct and corpuscle, Immunohistochemistry, Drug delivery
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